Journal: bioRxiv

Article Title: Longitudinal clonal tracking in humanized mice reveals sustained polyclonal repopulation of gene-modified human-HSPC despite vector integration bias

doi: 10.1101/2020.08.21.261537

Figure Lengend Snippet: a ) Diagram showing Anti-HIV-EGFP-WT and control mCherry-H1/H5 vectors having WT, H1, or H5 LTR-index and strategy for VIS assay with LTRi-seq. LTR index sequences are shown in magenta box. b ) Hu-BLT mouse model: Fetal liver CD34+ cells were separately transduced with either anti-HIV or control vectors and transduced cells were mixed 1:1 for transplant. The mix of transduced cells was transplanted in myeloablated NSG mice with a fetal thymus tissue implant. c ) Stacked bar plot showing clonal frequencies of VIS in BM of hu-BLT mouse. Clones from mCherry-H1 and EGFP-WT cells were identified by corresponding LTR barcodes. In the stacked bar plot, each band represents a unique VIS (HSPC clone) and thickness of the band shows clonal frequency or abundance of that HSPC clone. Percentage of mCherry+ or EGFP+ cells within human cell (hCD45+) population are shown on top of the corresponding stacked-bar. d ) Plot showing Pearson’s r for correlations of mCherry-H1 (red dots) and EGFP-WT (green dots) VIS clonal profiles between unamplified DNA replicates and replicates of MDA-amplified DNA samples for different cell numbers. e ) Experimental protocol for longitudinal clonal tracking in humanized BLT mice. f ) Scatter plot showing VIS clonal frequencies between unamplified whole blood DNA and two replicates of MDA-amplified DNA from 25µl blood at week 19 (r= Pearson’s r, diagonal line is r=1) for m599 and m598. Clonal frequency of mCherry-H5 (red dots) and EGFP-WT (green dots) VIS clones in unamplified DNA samples (y-axis) and MDA replicates (x-axis).

Article Snippet: The following monoclonal antibodies with fluorochromes were used: human CD45-eFluor 450 (HI30, eBioscience), CD3-APC-H7 (SK7: BD Pharmingen), and CD19-BV605 (HIB19: BioLegend).

Techniques: Transduction, Clone Assay, Amplification

Journal: Nature cell biology

Article Title: The ITIM-containing receptor LAIR1 is essential for acute myeloid leukemia development

doi: 10.1038/ncb3158

Figure Lengend Snippet: A, MV4-11 cells (1×10 6 cells) were infected with virus designed to express GFP and either scrambled shRNA or shRNA 226. GFP + cells were collected and transplanted into NSG mice (n = 7 mice) one day post-infection. Left panels show representative flow cytometry plots indicating decreased BM engraftment of MV4-11 cells treated with shRNA targeting lair1 . Staining with anti-human CD45 and anti-LAIR1 antibodies confirmed engraftment was from transplanted human leukemia cells. The percentages of each population are indicated in red numbers, and the median fluorescent intensity of LAIR1 expression is indicated in black numbers. The panel on the right plots percentages of GFP + cells in BM, spleen, liver, and PB at 1 month after transplantation (mean ± s.e.m., Student's t-test; n=7 samples, BM *** p < 0.0001; Spleen ** p = 0.0004; Liver * p = 0.0233; PB * p = 0.01471). B, Comparison of the sizes of spleens of the mice transplanted with control MV4-11 leukemia cells or cells expressing shRNA targeting lair1 (mean ± s.e.m., Student's t-test; n=7 samples, ** p = 0.0028). C, GFP + 697 cells infected by scrambled shRNA or shRNA 226 virus were collected, and 5×10 5 cells were transplanted into NSG mice (n = 5 mice for Scramble, n = 7 mice for lair1 null). Left are representative flow cytometry plots showing the decreased BM engraftment of lair1- knockdown 697 cells. Human CD45 and LAIR1 antibody staining confirmed engraftment was from transplanted human leukemia cells. The percentages of each population are indicated in red numbers, and the median fluorescent intensity of LAIR1 expression is indicated in black numbers. Shown on the right are percentages of GFP + cells in BM, spleen, liver, and PB at 1 month after transplantation (mean ± s.e.m., Student's t-test; n = 5 mice for Scramble, n = 7 mice for lair1 null, BM ** p = 0.0004; Spleen ** p = 0.0002; Liver *** p <0.0001; PB * p = 0.0057). D, Comparison of the sizes of livers of the mice transplanted with control or lair1 -knockdown 697 leukemia cells (mean ± s.e.m., Student's t-test; n = 5 mice for Scramble, n = 7 mice for lair1 null, * p = 0.0048). E, Summary of the effects of lair1 silencing in the indicated human leukemia cell lines on inhibition of cell growth in vitro and xenograftment in NSG mice.

Article Snippet: For analysis of human hematopoietic engraftment in NSG mice, we followed our previously published protocol , and used anti-human LAIR1 (clone 342219, R&D Systems) and anti-human CD45-PE (mouse, HI30, BD Pharmingen, 1:50 dilution) to quantify the total human AML engraftment.

Techniques: Infection, shRNA, Flow Cytometry, Staining, Expressing, Transplantation Assay, Inhibition, In Vitro

Journal: Nature cell biology

Article Title: The ITIM-containing receptor LAIR1 is essential for acute myeloid leukemia development

doi: 10.1038/ncb3158

Figure Lengend Snippet: A, Expression of lair1 mRNA differs significantly in BM and PB of AML patients (n = 7 BM or 19 PB respectively) from those in normal samples (n = 10 BM or 9 PB; mean ± s.e.m., Student's t-test; BM * p = 0.0329; PB * p = 0.0421). B, Representative flow cytometry plots showing different LAIR1 expression patterns in normal mononuclear cells (Cord blood) and primary AML cells (type 1 and type 2). A representative plot of a flow cytometry analysis (from at least 3 similar images) of an type 1 sample shows that cells are present that express both high and low levels of LAIR1. Type 2 samples mainly contain LAIR1 high cells. Data on these primary AML samples are listed in sTable 3. C, Summary of percentages of hCD45 + cells in PB of NSG mice transplanted with LAIR1 low or LAIR1 high primary human AML cells from 2 patient samples at 4 months after transplantation (mean ± s.e.m., Student's t-test, n= 5 mice, *** p < 0.0001). D&G, Expression of shp-1 or camk1 mRNA negatively correlates with the overall survival of AML patients. n = 82 samples for high and n = 83 samples for low (TCGA database); (D) p =0.0205; (G) p =0.0037, log-rank test). E&H, Treatment with shRNAs targeting shp-1 or camk1 inhibited the growth of MV4-11 cells. F&I, Increased apoptosis in shp-1 -silenced (by shRNA 698) or camk1 -silenced MV4-11 cells (mean ± s.e.m., Student's t-test; n =3 samples, (F) Day 3 *p =0.0324; Day 9 ** p = 0.0019; (I) Day 3 *p =0.0302; Day 9 ** p = 0.0031). J, Endogenous SHP-1 and CAMK1 expression was silenced with shRNAs (226, 277) in MV4-11 cells as determined by western blotting at 72 hours after shRNA-encoding lentiviral infection. K, Expression of lair1 , camk1 , and shp-1 in 165 AML patients (TCGA database) showed highly significant positive correlations. These correlations were also supported by clustering analysis. L, CREB inhibitor XX15 treatment inhibited the growth of MV4-11 cells. M, Flow cytometry analysis of apoptosis of MV4-11, U937, and 697 cells after one day of treatment with XX15 (mean ± s.e.m., Student's t-test; n =3 samples, MV4-11 *p =0.0411; U937 ** p = 0.0216; 697 *p =0.0294). In Fig. 8E, H, and L, data from one experiment with n=3 technical replicate samples are shown. The experiment was repeated 3 times with similar results.

Article Snippet: For analysis of human hematopoietic engraftment in NSG mice, we followed our previously published protocol , and used anti-human LAIR1 (clone 342219, R&D Systems) and anti-human CD45-PE (mouse, HI30, BD Pharmingen, 1:50 dilution) to quantify the total human AML engraftment.

Techniques: Expressing, Flow Cytometry, Transplantation Assay, shRNA, Western Blot, Infection

Journal: iScience

Article Title: Peripheral immunophenotyping reveals lymphocyte stimulation in healthy women living with hereditary breast and ovarian cancer syndrome

doi: 10.1016/j.isci.2024.109882

Figure Lengend Snippet:

Article Snippet: anti-human-CD45 (HI30) - 110Cd , Standard BioTools , Cat. No.: 3110001B.

Techniques: Recombinant, DNA Extraction, Staining, Blocking Assay, Mass Cytometry, Software

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A) Flow cytometry dot plot showing CD45 phosphatase activity versus CD27 expression on gated CD19 + human peripheral B cells. (B and C) CD45 phosphatase activity (B) and CD45 surface expression (C) of CD27 − (blue) and CD27 + B cells (red). Numbers in histograms represent CD45 activity (pCAP-SP1) (B) or CD45 surface expression (C) as the mean fluorescence intensity (MFI) ratio of CD27 + /CD27 − B cells. Bottom graphs: pCAP-SP1 or CD45 surface expression (MFI) in CD27 + relative to CD27 − B cells. (D) CD45 expression versus CD45 phosphatase activity in gated CD27 + MBCs; CD45 hi and CD45 lo expression gates are shown. (E) CD45 phosphatase activity and (F) CD45 expression in CD27 + MBCs expressing low (blue open histogram) or high (red open histogram) levels of surface CD45 compared to CD27 − B cells (filled blue histogram). Graphs show pCAP-SP1 or CD45 MFI relative to CD27 − B cells. n = 12. Related to . ****p < 0.0001.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Flow Cytometry, Activity Assay, Expressing, Fluorescence

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A and B) CD45 phosphatase activity (A) and CD45 surface expression (B) in naive B cells (left panels) and MBCs (right panels), control (CTR) treated (filled histogram) or CD40L (open histograms). (C) CD45 phosphatase activity (upper panel, n = 13), panCD45 surface expression (middle panel, n= 7), and CD45 mRNA levels (lower panel, n = 3) of naive B cells (blue) and MBCs (red), CTR or CD40L treated. Graphs show pCAP-SP1 (upper panel) or CD45 (middle panel) MFI values relative to naive B cells. Lower graph: Quantitative real-time PCR of CD45 mRNA normalized to POLR2A expressed as RQ (relative quantity). (D and F) Activated BCR signaling kinases pSyk (D) and pErk (F) upon BCR cross-linking in naive B cells (left panel) or MBC (right panel) in CTR+vehicle (VEH) (filled histograms), CD40L+VEH (open histograms), or CD40L+CD45 inhibitor (open dotted histograms). (E and G) Graphs show pSyk (n= 9) (E) and pErk (n = 7) (G) MFI values relative to CTR naive B cells. (H and I) Real-time quantitative PCR analysis of SYK (H) and ERK (I) mRNA relative to POLR2A expressed as RQ (n = 3). (J) Purified human B cells were stimulated for 1 h at 37 ◦ C before CD45 activity was measured. Graph depicts % CD45 activity + B cells in CTR or CD40L-stimulated B cells (n= 4). Related to . **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Activity Assay, Expressing, Control, Real-time Polymerase Chain Reaction, Purification

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A–C) Flow cytometry dot plots showing gating of peripheral blood B cell subsets. (D and E) CD45 phosphatase activity (D) and panCD45 surface expression (E) in human MBC and ASC subpopulations (red) compared to naive B cells (blue). Numbers in the histograms represent MFI ratio relative to naive B cells. (F and G) Graphs show pCAP-SP1 (F) and panCD45 surface expression (G) MFI values relative to naive B cells (n= 12). (H) Human BM CD19 − CD38 hi CD138 + (left panel) and CD19 + CD138 +/− CD38 + (right panel) previously shown to contain LLPCs and SLPCs, respectively. (I) CD45 phosphatase activity (left panel), CD45 surface expression (middle panel), and BLIMP1 expression (right panel) in LLPCs (red) and SLPCs (blue) relative to mature BM B cells (CD19 + SSC lo CD45 hi ) obtained from the same donor (gray). (J) Graphs show fold change in MFI of CD45 phosphatase activity (left panel) and CD45 surface expression (right panel) from human BM PCs relative to mature BM B cells from the same healthy donor (n = 4). Related to . *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Flow Cytometry, Activity Assay, Expressing

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A) Flow cytometry plots show gated CD38 hi CD138 hi ASCs (upper panel) and CD45 activity versus CD138 expression (lower panel) in the presence of Th signals and CD45 inhibitor as indicated. (B) Graph shows % CD38 hi CD138 hi ASC B cells (n= 8). (C) Histograms show the CD45-dependent regulation of ASC-associated markers and transcription factors. CTR+VEH (gray), CD40L+VEH (blue), CD40L+CD45 inhibitor (red). (D) Human peripheral B cells differentiated toward ASCs in the presence of CTR siRNA (upper panels) or CD45 siRNA (lower panels). (E) Graph shows % IRF4 + BLIMP1 + ASCs upon delivery of CTR or CD45 siRNA (n = 5). (F) Flow cytometry histograms show expression of CD45 activity (upper left panel), CD45 surface expression (upper right panel), BLIMP1 (lower left panel), and IRF4 (lower right panel) in purified B cells differentiated toward ASCs in the presence of CTR siRNA (gray) or CD45 siRNA (blue). Related to . *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Flow Cytometry, Activity Assay, Expressing, Purification

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A) Flow cytometry plots show IRF4 and BLIMP1 expression on gated live cells with increasing levels of CD40L. CD45 inhibitor was used at 1.25 µM. (B) Graph shows % IRF4 + BLIMP1 + B cells in naive (blue) and memory (red) B cell cultures. Black asterisks represent significant differences between naive B cells and MBCs. Blue and red asterisks represent significant differences in naive B cells or MBCs stimulated with increasing concentrations of CD40L, respectively (n=9). (C) CD45 phosphatase activity in naive B cells (blue) and MBCs (red) upon incremental concentrations of CD40L. (D) Graph shows fold increase in MFI of CD45 phosphatase activity relative to naive unstimulated B cells (n = 9). (E) Flow cytometry plots show co-expression of CD45 phosphatase activity and expression of PC transcription factors IRF4 and BLIMP1 in naive B cells and MBCs upon provision of Th signals or CTR-treated B cells. (F) Flow cytometry plot showing co-expression of pErk and IRF4 in MBCs differentiated toward ASCs. (G) Dot plot shows IRF4 + BLIMP1 + ASCs (left panel) backgating to IRF4 + pErk + cells (red) compared to overall MBCs (blue) (middle panel). Right panel: Histogram shows expression of pErk in IRF4 + BLIMP1 + ASCs (red) compared to total MBCs (blue). (H) Graph shows pErk MFI values in MBCs and IRF4 + BLIMP1 + ASCs (n = 6). Related to – . *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Flow Cytometry, Expressing, Activity Assay

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A) Representative dot plots showing LN GC B cells (B220 + GL7 + CD38 − ) and high- and low-affinity HA-reactive cells; HA binding versus IgG expression is shown. (B) Histograms show CD45 activity (left) and IRF4 expression (right) in GC low- (blue-filled histograms) and high-affinity (red) B cells. (C) BM PCs gated as Blimp1 + CD138 + cells (green) overlayed on histograms of low- (blue) and high-affinity (red) GC B cells showing CD45 activity (left) and Blimp1 (middle) and CD138 (right) expression levels. (D) Dot plots show BM PC gate from forward and side scatter (F/SSC)-gated cells (left) and from B220 + SSC lo -gated cells (right). (E) PCs were backgated on dot plots depicting CD45 activity and Blimp1 (left) and CD45 activity and IRF4 (right); PCs are depicted as red dots, BM B220 + B cells are represented in blue. (F) PCs (red) and B220 + B cells (blue) were analyzed for CD45 activity (left) and HA binding (right). (G and H) Graphs show MFI of CD45 activity of low-affinity (LoAff) and high-affinity (HiAff) GC B cells and BM PCs relative to B220 + B cells within the same sample (LN or BM). (I) Graph shows MFI ratio of HA normalized to IgG. Data are representative of two experiments including seven immunized mice in addition to immunization and staining controls. Related to . *p < 0.05, **p < 0.01.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Binding Assay, Expressing, Activity Assay, Staining

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: (A) Flow cytometry histogram showing surface staining of Galectin-1 in naive (gray), memory (blue), and PCs (red) in human peripheral blood B cells (left panel). Isotype control is shown by gray-dotted histogram. Graph shows fold increase in surface Galectin-1 staining (MFI) relative to naive B cells (n = 4). Contour plot depicts CD45 activity versus Galectin-1 surface staining in B cells (blue) and PCs (CD138 hi CD38 hi ) (red) backgated to CD45 activity hi Galectin-1 hi cells (right panel). (B) Histograms show CD45 activity (left panel), Galectin-1 staining (middle panel), and MEM-55 staining (right panel) in CTR- or NA-treated B cells. Graphs show fold increase in MFI of CD45 phosphatase activity (left lower panel) and Galectin-1 staining (right lower panel) relative to CTR-treated B cells (n = 6). (C) Upper panels: Dot plots show expression of IRF4 and BLIMP1 in naive B cells and MBCs differentiated toward ASCs in the presence of 10 and 100 µM OTX008 or VEH. Graph shows % IRF4 + BLIMP1 + B cells in MBC cultures. Lower panels: Galectin-1 surface expression (left panel) and CD45 phosphatase activity (right panel) in VEH-treated (blue histogram) and OTX-treated (blue dotted histogram) MBCs. Graphs show MFI values of Galectin-1 staining (left) and CD45 activity (right) (n = 5). (D) Flow cytometry dot plots show CD45 activity versus Galectin-1 surface staining in the presence of medium or rhGAL-1 (left panels). Histogram shows CD45 phosphatase activity of Galectin-1 hi B cells with rhGAL1 (blue) and total B cells (gray) (middle panel). Graph shows MFI values of CD45 activity in total B cells (gray histogram) and GAL-1 hi (blue open histogram) B cells (right panel) (n = 6). (E) Representative localization of Galectin-1 relative to pSyk, pCAP-SP1, and CD45 in human B cells. The panels (left) present signals from the individual fluorescence detectors, and the center image is a merge of all four channels. The graph (right) shows the average (z axis) fluorescence intensity for each (x axis) pixel number in the indicated area (below graph and dotted area on center figure). (F) CoIP of CD45 of B cell lysates and immunoblotted with anti-CD45 (left) or anti-Galectin-1 (right). (G) Flow cytometry histogram showing CD45 phosphatase activity in gated live Raji B cells with CRISPR-Cas9 knockdown of CD45 (blue), Galectin-1 (green), and wild-type (red). Related to and . *p < 0.05, **p < 0.01.

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Flow Cytometry, Staining, Control, Activity Assay, Expressing, Fluorescence, CRISPR, Knockdown

Journal: Cell reports

Article Title: Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity

doi: 10.1016/j.celrep.2021.109525

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: PO Mouse monoclonal anti human CD45 (clone HI30) , ThermoFisher , Cat# MHCD4530; RRID:AB_10376143.

Techniques: Negative Control, Recombinant, Purification, Staining, Gene Expression, Lysis, Immunoprecipitation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Blocking Assay, Cell Isolation, Software, Microscopy

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet: Surface antibody master mix

Article Snippet: Anti-Human CD45 (HI30)-89Y—100 Tests (1:100) , Fluidigm , Cat#3089003B.

Techniques:

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet: Clean up gating strategy used for identifying of viable, CD45 + CD138 - single cell population using gaussian parameters and 191Ir/193Ir biaxial plots The single cells were gated against bi-axial plots versus CD45 and CD138 to confirm the population of interest for downstream analysis.

Article Snippet: Anti-Human CD45 (HI30)-89Y—100 Tests (1:100) , Fluidigm , Cat#3089003B.

Techniques:

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet: CD45 expression level, as it is a phosphatase, is expressed in different levels on progenitor/stem cells and is donor dependent

Article Snippet: Anti-Human CD45 (HI30)-89Y—100 Tests (1:100) , Fluidigm , Cat#3089003B.

Techniques: Expressing

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet: Cleaned, CD45 hi population manually gated to define lineage populations

Article Snippet: Anti-Human CD45 (HI30)-89Y—100 Tests (1:100) , Fluidigm , Cat#3089003B.

Techniques:

Journal: STAR Protocols

Article Title: Mass cytometry staining for human bone marrow clinical samples

doi: 10.1016/j.xpro.2022.101163

Figure Lengend Snippet:

Article Snippet: Anti-Human CD45 (HI30)-89Y—100 Tests (1:100) , Fluidigm , Cat#3089003B.

Techniques: Recombinant, Saline, Red Blood Cell Lysis, Staining, Blocking Assay, Antibody Labeling, Software, Cell Counting, Cytometry

Journal: Cell Reports Medicine

Article Title: Multi-omic longitudinal study reveals immune correlates of clinical course among hospitalized COVID-19 patients

doi: 10.1016/j.xcrm.2023.101079

Figure Lengend Snippet:

Article Snippet: Anti-Human CD45 (HI30)-154Sm antibody , Fluidigm , Cat#3154001B.

Techniques: Purification, Produced, Virus, Clinical Proteomics, Recombinant, Ligation, Blocking Assay, Antibody Labeling, Staining, Binding Assay, Magnetic Beads, Sequencing, DNA Library Preparation, Cloning, Targeted Proteomics, Whole Genome Amplification, Plasmid Preparation, Software, Real-time Polymerase Chain Reaction, Hydrophilic Interaction Liquid Chromatography, Sample Prep, Transferring, Sonication

Journal: Immunity

Article Title: Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization

doi: 10.1016/j.immuni.2018.06.009

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-human CD45 , Fluidigm , HI30, cat#3156010B.

Techniques: Antibody Labeling, Virus, Recombinant, Control, Enzyme-linked Immunosorbent Assay, Software